434D
5'-R(*UP*AP*GP*CP*UP*CP*C)-3', 5'-R(*GP*GP*GP*GP*CP*UP*A)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.300, 28.900, 28.900 |
| Unit cell angles | 110.50, 96.90, 96.90 |
Refinement procedure
| Resolution | 15.000 - 1.160 |
| Rwork | 0.124 |
| R-free | 0.18000 * |
| Structure solution method | SIRAS |
| Starting model (for MR) | CANONICAL A-FORM RNA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.170 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.099 | 0.540 |
| Number of reflections | 23758 | |
| <I/σ(I)> | 12.6 | 1.5 |
| Completeness [%] | 88.4 | 75.7 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 21 * | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE | ||
| 2 | 1 | 1 | SODIUM CACODYLATE | ||
| 3 | 1 | 1 | SRCL2 | ||
| 4 | 1 | 1 | LICL | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 2 | 1 | drop | spermine tetra-HCl | 12 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | MPD | 10 (%(v/v)) | |
| 5 | 1 | drop | RNA | 0.5 (mM) | |
| 6 | 1 | reservoir | 35 (%(v/v)) |
