432D
D(GGCCAATTGG) COMPLEXED WITH DAPI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.616, 36.563, 52.961 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.890 |
| Starting model (for MR) | BD0006 USED AS STARTING MODEL FOR REFINEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.073 | 0.291 |
| Total number of observations | 24368 * | |
| Number of reflections | 4115 * | |
| <I/σ(I)> | 13.78 | 5.55 |
| Completeness [%] | 97.1 | 95.1 |
| Redundancy | 5.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.9 | 289 | pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 43 (mM) | ||
| 2 | 1 | drop | MPD | 3 (%) | |
| 3 | 1 | drop | spermine | 2 (mM) | |
| 4 | 1 | drop | ssDMA | 0.4 (mM) | |
| 5 | 1 | drop | DAPI | 0.2 (mM) | |
| 6 | 1 | reservoir | MPD | 50 (%) |
