430D
STRUCTURE OF SARCIN/RICIN LOOP FROM RAT 28S RRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-11-15 |
| Detector | ADSC |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 56.830, 56.830, 107.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.28 |
| Rwork | 0.280 |
| R-free | 0.33000 |
| Structure solution method | MAD PHASING METHOD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 30.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.043 | 0.217 |
| Total number of observations | 22518 * | |
| Number of reflections | 6036 * | |
| <I/σ(I)> | 32.5 | 1.9 |
| Completeness [%] | 92.7 * | 88.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 20 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | MOPS | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.0-3.2 (M) | |
| 5 | 1 | reservoir | MOPS | 50 (mM) | |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | spermine | 1 (mM) | |
| 8 | 1 | reservoir | 2 (mM) |
