429D
CRYSTAL STRUCTURE OF A LEADZYME; METAL BINDING AND IMPLICATIONS FOR CATALYSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 60.400, 60.400, 133.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.254 * |
| Rwork | 0.255 |
| R-free | 0.27400 * |
| Structure solution method | MAD PHASING USING 5 HEAVY ATOM DERIVATIVES (INCLUDING PB(II)) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.350 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.064 | 0.210 |
| Number of reflections | 4416 | |
| Completeness [%] | 96.6 | 55.4 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MAGNESIUM ACETATE | ||
| 2 | 1 | 1 | SODIUM CACODYLATE | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 0.8 (mM) | |
| 2 | 1 | drop | Na cacodylate | 10 (mM) | |
| 3 | 1 | reservoir | MPD | 20-25 (%) | |
| 4 | 1 | reservoir | spermine-HCl | 1 (mM) | |
| 5 | 1 | reservoir | 20 (mM) | ||
| 6 | 1 | reservoir | Na cacodylate | 50 (mM) |
