424D
5'-D(*AP*CP*CP*GP*AP*CP*GP*TP*CP*GP*GP*T)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1992-03-02 |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.539, 40.107, 40.469 |
| Unit cell angles | 82.62, 116.20, 80.68 |
Refinement procedure
| Resolution | 19.000 - 2.700 |
| R-factor | 0.216 * |
| Rwork | 0.211 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IDEAL B-DNA |
| RMSD bond length | 0.010 |
| RMSD bond angle | 16.400 * |
| Data scaling software | TEXSAN |
| Phasing software | ULTIMA |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.065 | 0.180 |
| Total number of observations | 5668 * | |
| Number of reflections | 5415 | 4608 * |
| Completeness [%] | 89.4 | 75.5 |
| Redundancy | 1.05 | 1.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | SODIUM CACODYLATE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | SODIUM CACODYLATE | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 2 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | spermine tetrachloride | 2 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 6 | 1 | drop | 5 (%(v/v)) | ||
| 7 | 1 | reservoir | MPD | 17 (%(w/v)) | |
| 8 | 1 | reservoir | sodium cacodylate | 100 (mM) |
