423D
5'-D(*AP*CP*CP*GP*AP*CP*GP*TP*CP*GP*GP*T)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-C |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-03 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | H 3 |
| Unit cell lengths | 64.067, 64.067, 44.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.600 - 1.600 |
| R-factor | 0.174 * |
| Rwork | 0.173 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IDEAL B-DNA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | ULTIMA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.052 | 0.235 |
| Total number of observations | 68426 * | |
| Number of reflections | 8888 | 8888 * |
| <I/σ(I)> | 50.8 | 4.9 |
| Completeness [%] | 98.7 | 95 |
| Redundancy | 7.7 | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 19 * | used to seeding * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | NA CACODYLATE | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.8 (mg/ml) | |
| 2 | 1 | drop | 70-80 (mM) | ||
| 4 | 1 | drop | spermine tetrachloride | 1-2 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 6 | 1 | drop | 6 (%(w/v)) | ||
| 7 | 1 | reservoir | MPD | 23 (%) | |
| 8 | 1 | reservoir | sodium cacodylate | 100 (mM) |
