420D
CRYSTAL STRUCTURE OF A 16-MER RNA DUPLEX WITH NON-ADJACENT A(ANTI).G(SYN) MISMATCHES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-27 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 |
| Unit cell lengths | 42.530, 42.530, 128.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.29600 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 * |
| Data reduction software | R-AXIS (IIC V. 2.1) |
| Data scaling software | MSC |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.270 | |
| High resolution limit [Å] | 1.820 | 1.900 * |
| Rmerge | 0.073 | |
| Number of reflections | 5521 * | |
| Completeness [%] | 81.0 * | 52.5 * |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | NA HEPES | ||
| 5 | 1 | 1 | PEG 400 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 1 (mM) | |
| 2 | 1 | drop | sodium HEPES | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | PEG400 | 5 (%) | |
| 6 | 1 | reservoir | PEG400 | 30 (%) |
