Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1990-12-15 |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 38.490, 32.300, 38.760 |
| Unit cell angles | 90.00, 117.56, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | RIGAKU |
| Data scaling software | RIGAKU |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 3556 |
| Completeness [%] | 90.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 10 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA tridecamer | 0.5 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 6.7 (mM) | |
| 3 | 1 | drop | sodium phosphate | 1.3 (mM) | |
| 4 | 1 | drop | 13.3 (mM) | ||
| 5 | 1 | drop | 3.3 (mM) | ||
| 6 | 1 | drop | spermine-3HCl | 3.7 (mM) | |
| 7 | 1 | drop | PEG400 | 0.8 (%) | |
| 8 | 1 | reservoir | PEG400 | 4 (%) |
