412D
DUPLEX [5'-D(GCGTA+TACGC)]2 WITH INCORPORATED 2'-O-METHYL-[TRI(OXYETHYL)] RIBONUCLEOSIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-11 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.640, 43.620, 45.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.650 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.22000 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.800 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.080 |
| Number of reflections | 6142 |
| Completeness [%] | 97.8 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | pH 6.30, VAPOR DIFFUSION, SITTING DROP | |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | pH 6.30, VAPOR DIFFUSION, SITTING DROP | |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | pH 6.30, VAPOR DIFFUSION, SITTING DROP | |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | pH 6.30, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 1.2 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 3 | 1 | drop | 8 (mM) | ||
| 4 | 1 | drop | spermine tetrahydrochloride | 20 (mM) | |
| 5 | 1 | reservoir | MPD | 40 (%(v/v)) |
