407D
STRUCTURAL BASIS FOR RECOGNITION OF A-T AND T-A BASE PAIRS IN THE MINOR GROOVE OF B-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.440, 30.510, 42.760 |
| Unit cell angles | 90.00, 120.47, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23600 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.030 |
| Total number of observations | 9893 * |
| Number of reflections | 3070 |
| Completeness [%] | 91.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 293 | pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CALCIUM ACETATE | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | TRIS | ||
| 4 | 1 | 2 | CALCIUM ACETATE | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | TRIS |
