402D
5'-R(*CP*GP*CP*CP*AP*GP*CP*G)-3'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | H 3 2 |
Unit cell lengths | 42.737, 42.737, 123.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.26300 |
RMSD bond length | 0.035 * |
RMSD bond angle | 0.867 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.300 | 2.300 * |
Rmerge | 0.044 | 0.340 * |
Total number of observations | 25094 * | |
Number of reflections | 2101 | |
Completeness [%] | 96.8 | 80.2 * |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | NACL | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE TETRAHYDROCHLORIDE | ||
5 | 1 | 1 | PRECIPITATING SOLUTION | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | NA CACODYLATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RNA | 2 (mM) | |
2 | 1 | reservoir | 20 (mM) | ||
3 | 1 | reservoir | 5 (mM) | ||
4 | 1 | reservoir | spermine tetrahydrochloride | 2 (mM) | |
5 | 1 | reservoir | MPD | 35 (%) | |
6 | 1 | reservoir | sodium cacodylate | 100 (mM) | precipitant |