3ZYH
CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH GALBG0 AT 1.5 A RESOLUTION
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 2 21 21 |
| 格子定数 [Å] | 40.559, 72.865, 133.723 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 49.264 - 1.501 |
| R因子 | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7l |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.108 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 66.860 | 1.590 |
| 分解能 [Å] (高) | 1.500 | 1.500 |
| Rmerge_l_obs | 0.030 | 0.070 |
| 独立反射数 | 61876 | |
| <I/σ(I)> | 34.5 | 14.8 |
| 完全性 [%] | 96.1 | 84.2 |
| 冗長性 | 3.5 | 2.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1.5 M AMMONIUM SULFATE AND 0.1 M SODIUM ACETATE PH 4.6. |
