3WOZ
Crystal structure of CLASP2 TOG domain (TOG3)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97893, 0.97921, 0.96399 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.896, 122.317, 86.514 |
Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.24252 |
Rwork | 0.241 |
R-free | 0.27941 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.898 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.220 |
High resolution limit [Å] | 2.200 | 5.700 | 2.180 |
Number of reflections | 56897 | ||
Completeness [%] | 99.5 | 97.5 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 8 | 277 | 10mM Tris, 50mM NaCl, 2mM DTT, pH 8.0, MICRODIALYSIS, temperature 277K |