3WFV

HIV-1 CRF07 gp41

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-16
Detector	ENRAF-NONIUS
Wavelength(s)	0.9793
Spacegroup name	H 3 2
格子定数 [Å]	46.920, 46.920, 185.051
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	20.102 - 1.800
R因子	0.2219
Rwork	0.220
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.226
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.102
分解能 [Å] (高)	1.800	1.800
Rmerge_l_obs		0.125
独立反射数	7326
<I/σ(I)>		17.5
完全性 [%]	95.8	95.76
冗長性		11.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	8% PEG6000, 0.1M Tris, 40% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K