3WD6
Crystal structure of Bombyx mori omega-class glutathione transferase in complex with GSH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 75.861, 89.894, 182.149 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.840 - 2.500 |
R-factor | 0.2104 |
Rwork | 0.208 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.088 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MERLOT |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.840 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 43880 | |
Completeness [%] | 98.4 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium iodide, and 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |