3V19
Forestalling insulin fibrillation by insertion of a chiral clamp mechanism-based application of protein engineering to global health
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.90020 |
Spacegroup name | H 3 |
格子定数 [Å] | 78.301, 78.301, 36.224 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 16.690 - 2.000 |
Rwork | 0.208 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rwe |
結合角の平均二乗偏差(RMSD) [度] | 0.008 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 16.690 | 2.070 |
分解能 [Å] (高) | 2.000 | 2.000 |
Rmerge_l_obs | 0.047 | 0.315 |
独立反射数 | 5515 | |
<I/σ(I)> | 43.5 | 6.57 |
完全性 [%] | 98.4 | 100 |
冗長性 | 5.2 | 5.1 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION | 8 | 298 | 0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.0, VAPOR DIFFUSION, temperature 298K |