3U1U
Crystal structure of RNA polymerase-associated protein RTF1 homolog Plus-3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Rigaku FR-E |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-17 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.479, 50.369, 69.776 |
Unit cell angles | 90.00, 103.37, 90.00 |
Refinement procedure
Resolution | 28.540 - 1.800 |
R-factor | 0.2036 |
Rwork | 0.202 |
R-free | 0.23490 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.355 |
Data reduction software | XDS |
Data scaling software | SCALA (CCP4_3.3.16) |
Phasing software | SHELX |
Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.076 | 0.020 | 0.890 |
Total number of observations | 7002 | 30390 | |
Number of reflections | 29882 | ||
<I/σ(I)> | 18.0335 | ||
Completeness [%] | 99.9 | 99.16 | 99.51 |
Redundancy | 7.2 | 7.06 | 7.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffusion | 5.5 | 291 | 25% PEG-3350, 0.2M ammonium sulfate, 0.1M sodium cacodylate, pH 5.5, sitting drop vapor diffusion, temperature 291K |
1 | sitting drop vapor diffusion | 5.5 | 291 | 25% PEG-3350, 0.2M ammonium sulfate, 0.1M sodium cacodylate, pH 5.5, sitting drop vapor diffusion, temperature 291K |