3Q27
Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 57.511, 49.951, 59.342 |
| 格子定数 [度] | 90.00, 92.19, 90.00 |
精密化法
| 残基 | 59.299 - 1.302 |
| R因子 | 0.1323 |
| Rwork | 0.131 |
| R-free | 0.15590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.164 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.4_486) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 80.000 | 80.000 | 1.350 |
| 分解能 [Å] (高) | 1.300 | 2.800 | 1.300 |
| Rmerge_l_obs | 0.078 | 0.050 | 0.568 |
| 独立反射数 | 82058 | ||
| <I/σ(I)> | 16 | ||
| 完全性 [%] | 99.6 | 96.1 | 99.9 |
| 冗長性 | 6.2 | 6.2 | 5.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 290 | 0.1M bicine pH 9.0, 2.4 M ammonium sulfate, vapor diffusion, hanging drop, temperature 290K |
