3NZE
The crystal structure of a domain of a possible sugar-binding transcriptional regulator from Arthrobacter aurescens TC1.
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 41.337, 86.286, 64.641 |
| 格子定数 [度] | 90.00, 94.75, 90.00 |
精密化法
| 残基 | 36.000 - 1.697 |
| R因子 | 0.1698 |
| Rwork | 0.168 |
| R-free | 0.19910 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.026 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 36.000 | 1.730 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.094 | 0.361 |
| 独立反射数 | 49143 | |
| <I/σ(I)> | 14.8 | 2 |
| 完全性 [%] | 98.1 | 84.6 |
| 冗長性 | 3.2 | 2.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 10% 2-propanol, 0.1M MES, 0.2M calcium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
