3NGO

Crystal structure of the human CNOT6L nuclease domain in complex with poly(A) DNA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9796
Spacegroup name	P 32 2 1
格子定数 [Å]	77.373, 77.373, 167.177
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	50.000 - 2.200
R因子	0.21718
Rwork	0.215
R-free	0.26314
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.016
結合角の平均二乗偏差(RMSD) [度]	1.629
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.240
分解能 [Å] (高)	2.200	2.200
Rmerge_l_obs	0.066	0.418
独立反射数	30217
<I/σ(I)>	52.7	4.7
完全性 [%]	99.7	99.2
冗長性	10.5	9.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M Hepes, pH 7.5, 1.1M ammonium tartrate, 0.2M NDSB-201, VAPOR DIFFUSION, HANGING DROP, temperature 291K