3M06
Crystal Structure of TRAF2
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | H 3 |
| 格子定数 [Å] | 150.706, 150.706, 86.728 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 36.130 - 2.670 |
| R因子 | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m0a |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.236 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.690 |
| 分解能 [Å] (高) | 2.640 | 7.050 | 2.640 |
| Rmerge_l_obs | 0.068 | 0.040 | 0.376 |
| 独立反射数 | 20824 | ||
| <I/σ(I)> | 13.4 | ||
| 完全性 [%] | 92.2 | 99.4 | 45.5 |
| 冗長性 | 5.8 | 5.8 | 5.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 8% PEG3350, 6% Tacsimate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
