3LP6
Crystal Structure of Rv3275c-E60A from Mycobacterium tuberculosis at 1.7A resolution
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 21 2 |
| 格子定数 [Å] | 145.696, 145.696, 58.384 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 45.558 - 1.702 |
| R因子 | 0.1693 |
| Rwork | 0.168 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1O4VA |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.993 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6_292)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.760 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.108 | 0.463 |
| 独立反射数 | 63825 | |
| <I/σ(I)> | 13.4 | 2.8 |
| 完全性 [%] | 92.1 | 85 |
| 冗長性 | 18.6 | 8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 298 | 0.1M Na acetate pH5.4, 1.7M Na formate, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
