3KYZ

The crystal structure of the sensor domain of two-component sensor PfeS from Pseudomonas aeruginosa PA01

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97932
Spacegroup name	I 21 21 21
格子定数 [Å]	29.920, 95.468, 103.565
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	35.000 - 1.497
R因子	0.1527
Rwork	0.151
R-free	0.18110
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	0.957
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	35.000	1.530
分解能 [Å] (高)	1.500	1.500
Rmerge_l_obs	0.095	0.731
独立反射数	24347
<I/σ(I)>	41.4	2.5
完全性 [%]	99.7	97.4
冗長性	8.5	7.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	5.5	297	0.2M MgCl2, 0.1M Bis-Tris, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K