3KYZ
The crystal structure of the sensor domain of two-component sensor PfeS from Pseudomonas aeruginosa PA01
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97932 |
Spacegroup name | I 21 21 21 |
格子定数 [Å] | 29.920, 95.468, 103.565 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 35.000 - 1.497 |
R因子 | 0.1527 |
Rwork | 0.151 |
R-free | 0.18110 |
Structure solution method | SAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
結合角の平均二乗偏差(RMSD) [度] | 0.957 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 35.000 | 1.530 |
分解能 [Å] (高) | 1.500 | 1.500 |
Rmerge_l_obs | 0.095 | 0.731 |
独立反射数 | 24347 | |
<I/σ(I)> | 41.4 | 2.5 |
完全性 [%] | 99.7 | 97.4 |
冗長性 | 8.5 | 7.2 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 0.2M MgCl2, 0.1M Bis-Tris, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |