3JR9

Crystal structure of Fis bound to 27 bp optimal binding sequence F2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	42.960, 91.690, 154.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.410 - 2.900
R-factor	0.224
Rwork	0.222
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eto
RMSD bond length	0.009
RMSD bond angle	1.503
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	3.000
High resolution limit [Å]	2.900	6.250	2.900
Rmerge	0.165	0.133	0.668
Number of reflections	14353
<I/σ(I)>	6.1
Completeness [%]	99.0	98.6	99.4
Redundancy	3.2	3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 36% PEG 400, vapor diffusion, hanging drop, temperature 277K