3IQF
Structure of F420 dependent methylene-tetrahydromethanopterin dehydrogenase in complex with methenyl-tetrahydromethanopterin
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.008 |
Spacegroup name | P 1 21 1 |
格子定数 [Å] | 103.400, 167.100, 122.500 |
格子定数 [度] | 90.00, 113.60, 90.00 |
精密化法
残基 | 112.500 - 2.100 |
R因子 | 0.16246 |
Rwork | 0.160 |
R-free | 0.20028 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qv9 |
結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
結合角の平均二乗偏差(RMSD) [度] | 1.631 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 112.500 | 2.100 |
分解能 [Å] (高) | 2.000 | 2.000 |
独立反射数 | 246709 | |
<I/σ(I)> | 14.9 | 2.7 |
完全性 [%] | 99.2 | 77.6 |
冗長性 | 4 | 77.6 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG400, 0.1M MES, 0.1M sodium acetate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |