3HDF

Crystal structure of truncated endolysin R21 from phage 21

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	2008-06-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9796
Spacegroup name	P 21 21 2
格子定数 [Å]	64.190, 109.673, 45.015
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	34.793 - 1.700
Rwork	0.200
R-free	0.23100
Structure solution method	SIRAS
結合長の平均二乗偏差(RMSD) [Å]	0.004
結合角の平均二乗偏差(RMSD) [度]	0.647
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX

Quality characteristics

	Overall	Inner shell	Outer shell
分解能 [Å] (低)	50.000	50.000	1.760
分解能 [Å] (高)	1.700	3.660	1.700
Rmerge_l_obs	0.038	0.020	0.372
独立反射数	35740
<I/σ(I)>	44.163
完全性 [%]	99.9	98.9	100
冗長性	6.1	5.8	6.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	290	100mM Bis-Tris propane, 2.5M sodium nitrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K