3H0L
Structure of trna-dependent amidotransferase gatcab from aquifex aeolicus
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0274,0.9804 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 127.482, 131.012, 154.668 |
| 格子定数 [度] | 90.02, 90.00, 89.91 |
精密化法
| 残基 | 40.500 - 2.300 |
| R因子 | 0.24169 |
| Rwork | 0.240 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2GI3 and 2G5H |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.396 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.500 | 2.350 |
| 分解能 [Å] (高) | 2.300 | 2.300 |
| Rmerge_l_obs | 0.060 | 0.347 |
| 独立反射数 | 405875 | |
| <I/σ(I)> | 11.5 | 1.8 |
| 完全性 [%] | 91.5 | 73.7 |
| 冗長性 | 1.9 | 1.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1:1 ratio of Protein solution (6-9mg/ml GatCAB,10mM HEPES, 50uM Zn acetate,10mM Asn, 0.6mM ATP) mix with well solution (10-12% PEG3350, 10mM Mg formate), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1:1 ratio of Protein solution (6-9mg/ml GatCAB,10mM HEPES, 50uM Zn acetate,10mM Asn, 0.6mM ATP) mix with well solution (10-12% PEG3350, 10mM Mg formate), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
