3GUW
Crystal Structure of the TatD-like Protein (AF1765) from Archaeoglobus fulgidus, Northeast Structural Genomics Consortium Target GR121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97893 |
Spacegroup name | P 1 |
Unit cell lengths | 40.069, 72.192, 89.163 |
Unit cell angles | 89.87, 89.74, 82.73 |
Refinement procedure
Resolution | 19.810 - 3.200 |
R-factor | 0.243 |
Rwork | 0.241 |
R-free | 0.29200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXCD (& SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.087 | 0.245 |
Number of reflections | 32519 | |
<I/σ(I)> | 9.3 | 2.19 |
Completeness [%] | 97.5 | 98.1 |
Redundancy | 1.8 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1M sodium acetate, 1.5M (NH4)2SO4, and 15% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 291K |