3FWB
Sac3:Sus1:Cdc31 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.535, 60.062, 77.300 |
Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.19063 |
Rwork | 0.188 |
R-free | 0.23489 |
Starting model (for MR) | P21 crystal structure obtained as described in publication |
RMSD bond length | 0.009 |
RMSD bond angle | 1.091 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.069 | 0.431 |
Number of reflections | 16233 | |
<I/σ(I)> | 9.7 | 2.7 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M MES pH6.5, 16% PEG4K, 20% glycerol - see publication for complete details, VAPOR DIFFUSION, HANGING DROP, temperature 291K |