3EY8
Structure from the mobile metagenome of V. Pseudocholerae. VPC_CASS1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-07 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 38.851, 62.087, 98.055 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 26.228 - 1.600 |
| R因子 | 0.1579 |
| Rwork | 0.156 |
| R-free | 0.18730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ey7 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 26.230 | 1.690 |
| 分解能 [Å] (高) | 1.600 | 1.600 |
| Rmerge_l_obs | 0.045 | 0.089 |
| 独立反射数 | 27068 | |
| <I/σ(I)> | 40.9 | 17.4 |
| 完全性 [%] | 84.5 | 38.9 |
| 冗長性 | 7 | 5.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 25% Peg 3350 0.2M LiSo4 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, temperature 294K |
