3EOC
Cdk2/CyclinA complexed with a imidazo triazin-2-amine
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 62 2 2 |
| 格子定数 [Å] | 182.977, 182.977, 213.687 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 20.000 - 3.200 |
| R因子 | 0.21585 |
| Rwork | 0.215 |
| R-free | 0.23836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvv |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.036 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 3.280 |
| 分解能 [Å] (高) | 3.200 | 3.200 |
| Rmerge_l_obs | 0.110 | 0.610 |
| 独立反射数 | 33305 | |
| <I/σ(I)> | 14.9 | 2.1 |
| 完全性 [%] | 98.9 | 99 |
| 冗長性 | 4.9 | 4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 1.5 M Sodium acetate, 100 mM Sodium cacodylate pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
