3EKI
Structural insights of the Mycoplasma hyorhinis protein Mh-p37: A putative thiamine pyrophosphate transporter
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9169 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 49.457, 67.586, 59.838 |
| 格子定数 [度] | 90.00, 106.28, 90.00 |
精密化法
| 残基 | 40.000 - 1.600 |
| Rwork | 0.183 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e78 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | SHELXL-97 |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 1.660 |
| 分解能 [Å] (高) | 1.600 | 1.600 |
| 独立反射数 | 47500 | |
| <I/σ(I)> | 0.115 | 3.1 |
| 完全性 [%] | 94.6 | 95.6 |
| 冗長性 | 2.7 | 2.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Batch | 4 | 298 | 0.1 M Ammonium Bromide, 0.1 M Citric Acid, 40% PEG 4000, under paraffin oil, pH 4.0, Batch, temperature 298K |
