3E78

Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mh-p37

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2008-05-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
格子定数 [Å]	50.445, 68.761, 60.024
格子定数 [度]	90.00, 108.04, 90.00

精密化法

残基	25.000 - 1.900
R因子	0.186
R-free	0.24000
Structure solution method	SIRAS
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.022
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	SHELXL-97

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	25.000	1.980
分解能 [Å] (高)	1.900	1.900
独立反射数	29894
<I/σ(I)>	16.3	5
完全性 [%]	96.2	93.2
冗長性	3.5	3.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Batch	3	298	0.1 M Ammonium Bromide, O.1 M Citric Acid, 40% PEG 4000, 9% N-decyl-beta-D-maltoside detergent , pH 3.0, Batch, temperature 298K