3E50
Crystal structure of human insulin degrading enzyme in complex with transforming growth factor-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-28 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0003 |
Spacegroup name | P 65 |
Unit cell lengths | 262.172, 262.172, 90.522 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.19274 |
Rwork | 0.191 |
R-free | 0.23133 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.982 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.094 |
Number of reflections | 157230 |
<I/σ(I)> | 23.6 |
Completeness [%] | 100.0 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |