3ZZZ
Crystal structure of a Raver1 PRI4 peptide in complex with polypyrimidine tract binding protein RRM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.480, 60.380, 61.060 |
| Unit cell angles | 90.00, 107.86, 90.00 |
Refinement procedure
| Resolution | 20.070 - 1.550 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zzy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (1.3.1) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.490 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 36184 | |
| <I/σ(I)> | 10.2 | 3 |
| Completeness [%] | 96.9 | 96.5 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE PAPER., pH 6.5 |
