3ZZY
Crystal structure of a Raver1 PRI3 peptide in complex with polypyrimidine tract binding protein RRM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.233, 60.606, 60.842 |
| Unit cell angles | 90.00, 107.51, 90.00 |
Refinement procedure
| Resolution | 29.010 - 1.400 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sjr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (1.3.1) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.320 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 50129 | |
| <I/σ(I)> | 15.4 | 5.3 |
| Completeness [%] | 98.9 | 96.1 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE PAPER., pH 6.5 |
