3ZZQ
Engineered 12-subunit Bacillus subtilis trp RNA-binding attenuation protein (TRAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 146.348, 146.348, 146.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.750 |
| R-factor | 0.17307 |
| Rwork | 0.173 |
| R-free | 0.19040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qaw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.051 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0058) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 51357 | |
| <I/σ(I)> | 42.9 | 6.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 25.4 | 22.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
