3ZZJ
Structure of an engineered aspartate aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.139, 154.200, 79.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.500 |
| R-factor | 0.21899 |
| Rwork | 0.217 |
| R-free | 0.26231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aaw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.030 | 0.150 |
| Number of reflections | 18589 | |
| <I/σ(I)> | 43.7 | 14.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.5 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.0 |
