3ZYY

Reductive activator for corrinoid,iron-sulfur protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	70.527, 91.191, 252.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.936 - 2.200
R-factor	0.222
Rwork	0.220
R-free	0.25800
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	1.836
Phasing software	AutoSol
Refinement software	PHENIX ((PHENIX.REFINE: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.510
Number of reflections	339176
<I/σ(I)>	16	2.7
Completeness [%]	99.6	99.7
Redundancy	13.6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.15 M MG-FORMATE, 15% (W/V) PEG 3350