3ZYL
Structure of a truncated CALM (PICALM) ANTH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-20 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.888, 121.082, 62.418 |
Unit cell angles | 90.00, 110.75, 90.00 |
Refinement procedure
Resolution | 35.509 - 1.700 |
R-factor | 0.1706 |
Rwork | 0.170 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hf8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.500 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.480 |
Number of reflections | 72568 | |
<I/σ(I)> | 9.8 | 2.1 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | CRYSTALS WERE GROWN IN SITTING DROPS CONTAINING EQUAL AMOUNTS (200-400 NL) OF PROTEIN (15 MG/ML IN 20 MM HEPES PH 7.4, 120 MM NACL, 4 MM DTT) AND RESERVOIR SOLUTION (15% V/V ETHANOL, 100 MM IMIDAZOLE PH 8.0, 200 MM MGCL2) AND EQUILIBRATED AGAINST 80 UL OF RESERVOIR SOLUTION AT 16C. CRYSTALS WERE CRYOPROTECTED BY BRIEF IMMERSION (5-60 S) IN RESERVOIR SOLUTION SUPPLEMENTED WITH 25% GLYCEROL AND WERE RAPIDLY CRYOCOOLED IN LIQUID N2 OR A 100 K STREAM OF N2 GAS. |