3ZY4
Crystal structure of POFUT1 apo-form (crystal-form-I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.440, 42.533, 96.924 |
| Unit cell angles | 90.00, 107.24, 90.00 |
Refinement procedure
| Resolution | 92.570 - 1.740 |
| R-factor | 0.23532 |
| Rwork | 0.235 |
| R-free | 0.25196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zy2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.110 | 0.280 |
| Number of reflections | 46747 | |
| <I/σ(I)> | 6.5 | 2.7 |
| Completeness [%] | 98.9 | 96.6 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
