3ZXU
Crystal structure of the Ctf19-Mcm21 kinetochore heterodimer from yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 239.036, 239.036, 179.487 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.492 - 3.700 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.20890 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.665 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.360 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.190 | 4.990 |
| Number of reflections | 31774 | |
| <I/σ(I)> | 13.9 | 0.7 |
| Completeness [%] | 97.2 | 85.2 |
| Redundancy | 18.8 | 16.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.1 | 293 | 7-10 % (W/V) POLYETHYLENE GLYCOL 8000, 14-20 % (V/V) ETHYLENE GLYCOL, 30 MM CACL2, 30 MM MGCL2, 100 MM BICINE/TRIS PH 8.1-8.5, 0.5 MM TCEP, TEMPERATURE 293 K. |
