3ZXG
lysenin sphingomyelin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.150, 37.200, 96.740 |
| Unit cell angles | 90.00, 104.03, 90.00 |
Refinement procedure
| Resolution | 50.380 - 3.120 |
| R-factor | 0.2404 |
| Rwork | 0.239 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zx7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.100 | 3.300 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 13456 | |
| <I/σ(I)> | 16.3 | 3.3 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 7.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BIS-TRIS PH 5.5, 0.2 M LI2SO4. |
