3ZXD
wild-type lysenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.910, 85.560, 108.810 |
| Unit cell angles | 98.88, 96.84, 90.04 |
Refinement procedure
| Resolution | 29.090 - 3.300 |
| R-factor | 0.2142 |
| Rwork | 0.213 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zx7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.360 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.160 | 0.460 |
| Number of reflections | 30603 | |
| <I/σ(I)> | 8.3 | 2.8 |
| Completeness [%] | 97.8 | 97.4 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1M MES, 1.6M MAGNESIUM SULFATE PH 6.5 |
