3ZXB

Wild type single insulin-like growth factor binding domain protein (SIBD-1) from Cupiennius salei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-08
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	62.247, 62.247, 169.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.366 - 2.550
R-factor	0.2257
Rwork	0.224
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zxc
RMSD bond length	0.009
RMSD bond angle	1.425
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.700
High resolution limit [Å]	2.550	2.550
Rmerge	0.070	0.100
Number of reflections	13082
<I/σ(I)>	23.9	2.98
Completeness [%]	99.7	99.4
Redundancy	7.7	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		1.4 M SODIUM CITRATE, 0.1 M TRIS, 2 % GLYCEROL, PH 8.