3ZXA

Structure and Assembly of Turnip Crinkle Virus I. X-ray Crystallographic Structure Analysis at 3.2 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-6
Temperature [K]	293
Detector technology	FILM
Detector	KODAK NO-SCREEN X-RAY FILM
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	348.800, 379.100, 397.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 3.200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TBSV
Data reduction software	OPTRONICS (P1000 ROTATING DRUM SCANNER)
Data scaling software	HARVARD (SOFTWARE)
Phasing software	COMBINE
Refinement software	BILDER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000
High resolution limit [Å]	3.200	3.200
Rmerge	0.180
Number of reflections	310000
Completeness [%]	73.0
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		THE METHYL MERCURY ADDUCT WAS OBTAINED BY BRINGING STOCK SOLUTION OF VIRUS (3.5% TCV (W/V) IN 0.01% SODIUM AZIDE,) TO 6 EQUIVALENT METHYL MERCURY/PROTEIN SUBUNIT BY ADDITION OF 15 MILLIMOLAR-METHYL MERCURY NITRATE AND INCUBATING FOR 1 H. CRYSTALLIZATION WAS THEN INITIATED BY ADDITION OF AN APPROXIMATELY EQUAL VOLUME OF SATURATED SODIUM CITRATE (PH 7.0) AND ALLOWED TO PROCEED UNDISTURBED FOR 2 TO 4 MONTHS. THE OPTIMUM CONCENTRATION OF SODIUM CITRATE REQUIRED TO PRODUCE LARGE CRYSTALS VARIED FROM EXPERIMENT TO EXPERIMENT BUT WAS GENERALLY IN THE RANGE 42 TO 46% SATURATED.