3ZX1
Multicopper oxidase from Campylobacter jejuni: a metallo-oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.810, 94.860, 50.380 |
| Unit cell angles | 90.00, 100.63, 90.00 |
Refinement procedure
| Resolution | 49.510 - 1.950 |
| R-factor | 0.16255 |
| Rwork | 0.160 |
| R-free | 0.20714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxw |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.534 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.510 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 32673 | |
| <I/σ(I)> | 14.9 | 3.5 |
| Completeness [%] | 99.4 | 97.3 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 32% PEG 3350, 0.2M MGCL2, 0.1M BIS-TRIS PROPANE PH5.5, 3% DIOXANE. |
