3ZWZ
Crystal structure of Plasmodium falciparum AMA1 in complex with a 39aa PfRON2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.150, 38.260, 70.750 |
| Unit cell angles | 90.00, 99.73, 90.00 |
Refinement procedure
| Resolution | 34.870 - 2.100 |
| R-factor | 0.16583 |
| Rwork | 0.164 |
| R-free | 0.20085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8v |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.528 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.150 | 0.470 |
| Number of reflections | 22041 | |
| <I/σ(I)> | 8.7 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG400, 100 MM TRIS-HCL PH 8.5, 200 MM TRI-SODIUM CITRATE. |
