3ZWL
Structure of eukaryotic translation initiation factor eIF3i complex with eIF3b C-terminus (655-700)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 126.469, 126.469, 105.741 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 109.530 - 2.200 |
| R-factor | 0.19166 |
| Rwork | 0.189 |
| R-free | 0.24706 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.882 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.700 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.140 | |
| Number of reflections | 49844 | |
| <I/σ(I)> | 10.2 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 0.1 M TRIS PH 8.8, 0.1 M LI2SO4, 30% PEG4000, 8% DIAMINOPENTANE |
