3ZWE
Structure of BambL, a lectin from Burkholderia ambifaria, complexed with blood group B epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.980, 49.140, 102.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.330 - 1.750 |
| R-factor | 0.21095 |
| Rwork | 0.208 |
| R-free | 0.26579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wz0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.622 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.350 |
| Number of reflections | 24165 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 96.0 | 79.9 |
| Redundancy | 3.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
